ABSTRACT
No abstract available.
Subject(s)
Child, Preschool , Female , Humans , Flow Cytometry , Hydroa Vacciniforme/diagnosis , Immunophenotyping , Lymphocytosis/complications , Lymphoma/diagnosis , Receptors, Antigen, T-Cell, gamma-delta/genetics , STAT3 Transcription Factor/genetics , Sequence Analysis, DNA , Skin/metabolism , T-Lymphocytes/metabolismABSTRACT
No abstract available.
Subject(s)
Aged , Humans , Male , Leukemia, Plasma Cell/complications , Leukocytosis , Lymph Nodes/pathology , Lymphoma, T-Cell/complications , Paraproteinemias/complications , Polymerase Chain Reaction , Receptors, Antigen, T-Cell, gamma-delta/genetics , Tomography, X-Ray ComputedABSTRACT
No abstract available.
Subject(s)
Female , Humans , Middle Aged , Antibodies, Monoclonal, Murine-Derived/therapeutic use , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , PAX5 Transcription Factor/metabolism , Bone Marrow/metabolism , Cyclophosphamide/therapeutic use , Doxorubicin/therapeutic use , Endoscopy, Digestive System , Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor , Genetic Loci , Liver/metabolism , Lymphocytes/cytology , Lymphoma, Large B-Cell, Diffuse/complications , Lymphoma, T-Cell, Peripheral/complications , Prednisone/therapeutic use , Receptors, Antigen, T-Cell, gamma-delta/genetics , Tomography, X-Ray Computed , Vincristine/therapeutic useABSTRACT
The patient was a 50-year-old woman who presented intermittent mild fever with elevated liver enzymes for 12 years. The liver biopsy showed diffuse portal and sinusoidal involvement of lymphoid cells with minimal atypia and epithelioid histiocytic granuloma formation. Subsequent bone marrow biopsy showed lymphomatous involvement. The lymphocytes infiltrating the liver were reactive for T-cell marker and showed TCR gamma gene rearrangement. The patient was diagnosed as primary peripheral T-cell lymphoma of the liver. Indolent clinical course and resemblance with hepatitis were considered to be a rare and unique feature of this case.
Subject(s)
Female , Humans , DNA, Neoplasm/analysis , Gene Rearrangement , Liver Neoplasms/diagnostic imaging , Liver Neoplasms/pathology , Liver Neoplasms/genetics , Lymphoma, T-Cell/diagnostic imaging , Lymphoma, T-Cell/pathology , Lymphoma, T-Cell/genetics , Middle Aged , Receptors, Antigen, T-Cell, gamma-delta/genetics , Tomography, X-Ray ComputedABSTRACT
Reduction in the background problems to improve the efficiency of nonradioactive labeling and detection procedure has been the focus of attention in the recent past for wider acceptance of the technique in nucleic acid research. We have achieved success in obtaining a relatively background free detection of single copy genes such as, T-cell receptor-delta (TCR-delta) and interleukin-2 receptor (L-2r) in human genome by using optimized concentrations of the digoxigenin labeled probes. Inclusion of such an optimization step for each probe before carrying out the actual hybridization experiment did not require any further modification in hybridization conditions and detection protocols as suggested earlier [Anal Biochem, 210 (1993) 235; Colloq Boehringer Mannheim, 2 (1991) 4.]
Subject(s)
Blotting, Southern , Luminescent Measurements , Digoxigenin/chemistry , Humans , Molecular Probes , Receptors, Antigen, T-Cell, gamma-delta/genetics , Receptors, Interleukin-2/geneticsABSTRACT
Se ha demostrado que los virus exógenos del tumor mamario murino (MMTV) transmitidos por leche, inducen la expresión de diferentes superantígenos en los huéspedes infectados. Cada uno de estos superantígenos es capaz de inducir la deleción clonal progresiva de las células T portadoras de determinados elementos Vß de su receptor (TCR). En este trabajo se describe la existencia de una alteración en el repertorio T de los ratones BALB/c de una colonia. Dicha alteración, transmitida por vía materna, involucra la deleción de las células T CD4+ que expresan las cadenas Vß2 y Vß14 del TCR y correlaciona con una alta incidencia de tumores de mama. Estos resultados indican la transmisión materna de un superantígeno(s), probablemente asociado a la presencia de virus MMTV en la leche